Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease and trauma. The enzyme catalysed reaction product phenylhydrazone can be measured at a colorimetric readout at 520 nm.

Functional Assay

Aspartate Transaminase

100 Assays

1137.50

Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. The assay is initiated with the enzymatic catalysis of glucose by glucose oxidase. The enzyme catalysed reaction products H2O2 react with the substrate, and can be measured at a colorimetric readout at 505 nm.

Functional Assay

Glucose (Tissue)

100 Assays

1400.00

Sucrase is the name given to a number of enzymes located in on the brush border of the small intestine that catalyze the hydrolysis of sucrose to fructose and glucose. The enzyme invertase, which occurs more commonly in plants, also hydrolyzes sucrose but by a different mechanism. The enzyme catalysed reaction products can be measured at a colorimetric readout at 540 nm.

Functional Assay

Sucrase (Plant)

100 Assays

1575.00

Sucrose phosphate synthase (SPS, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue. Fructose-6-phosphate is catalyzed by sucrose phosphate synthase to generate sucrose phosphate, and then react with resorcinol present a color change, have acharacteristic absorption peak at 480nm. The intensity of the product color, measured at 480 nm, is proportionate to the enzyme activity in the sample.

Functional Assay

Sucrose Phosphate Synthase

100 Assays

2677.50

Glutathione reductase (GR, EC 1.6.4.2) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to glutathione (GSH). This enzyme is essential for the GSH redox cycle which maintains adequate levels of reduced cellular GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. Glutathione Reductase Microplate Assay Kit measures GR activity by measuring the rate of NADPH oxidation. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. Since GR is present at rate limiting concentrations, the rate of decrease in the A340 is directly proportional to the GR activity in the sample.

Functional Assay

Glutathione Reductase

100 Assays

1312.50

Creatine Kinase (CK), also known as phosphocreatine kinase, is an enzyme that catalyzes the transfer of one phosphate group from ATP to creatine generating phosphocreatine, an important energy reservoir in muscle and brain tissue. CK is a dimeric protein made up of B (brain) and M (muscle) subunits. Three isoenzymes, CK-MM, CK-MB, and CK-BB, have been observed. CK levels are elevated in various pathological conditions including myocardial infarction, rhabdomyolysis, muscular dystrophy, and renal failure. Creatine Kinase Activity Microplate Assay Kit provides a simple and direct procedure for measuring CK levels in a variety of samples such as blood, serum, and plasma. In this reaction, phosphocreatine and ADP are converted to creatine and ATP. The generated ATP is used by hexokinase to phosphorylate glucose resulting in glucose-6-phosphate, which is oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase to produce NADPH and 6-phospho-D-gluconate, measured at 570 nm, proportionate to the CK activity present in the sample.

Functional Assay

Creatine Kinase

100 Assays

3071.25

In plants, the monodehydroascorbate reductase (MDAR) is an enzymatic component of the glutathione-ascorbate cycle that is one of the major antioxidant systems of plant cells for the protection against the damages produced by reactive oxygen species (ROS). The MDAR activity has been described in several cell compartments, such as chloroplasts, cytosol, mitochondria, glyoxysomes, and leaf peroxisomes. The assay is initiated with the enzymatic catalysis of the NADH by MDAR. NADH can be measured at a colorimetric readout at 340 nm.

Functional Assay

Monodehydroascorbate Reductase

100 Assays

2520.00

Glutamate Dehydrogenase (GDH) is a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to a-ketoglutarate and serves as a key link between anabolic and catabolic pathways. In mammals, GDH is subject to allosteric regulation and has high activity in liver, kidney, brain, and pancreas. GDH activity in serum can be used to differentiate between liver diseases due to liver inflammation, which do not show elevated serum GDH activity, and diseases that result in hepatocyte necrosis, which results in elevated serum GDH. The assay is initiated with the enzymatic catalysis of NH4+, α ketoglutaric acid and NADH by GDH. NADH can be measured at a colorimetric readout at 340 nm.

Functional Assay

Glutamate Dehydrogenase

100 Assays

2283.75