环球基因-

VSV-G-Tag Monoclonal Antibody(UMA00037)

Size	Price
50ul	499.00
100ul	799.00

Basic information

Catalog No.:UMA00037

Source:Mouse

Size:50ul/100ul

Clonality:Monoclonal

Concentration:1mg/ml

Isotype:IgG

Purification:The antibody was purified by immunogen affinity chromatography.

Useful information

Applications:

WB: 1:2000 - 1:5000
IF/IC: 1:200-1:500
IP: 1:100-1:200

Reactivity:

N/A

Specificity:

Recognizes C-terminal, internal, and N-terminal VSV-G-tag fusion proteins.

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of VSV-G-tag. The exact sequence is proprietary.

Description:

Mouse monoclonal antibody to VSV-G-tag

Uniprot:

BiowMW:

Refer to Figures

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage:

Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles.

Note:

For research use only, not for use in diagnostic procedure.

Alternative Name:

Data:

Western blot analysis of over-expressed VSV-G-tagged protein in 293T cell lysate.

Immunofluorescent analysis of VSV-G-tag staining in 293T cells transfected with a VSV-G-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation of VSV-G-tagged protein from HEK293T cells transfected with vector overexpressing VSV-G tag, using Anti-VSV-G-tag Antibody.

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