Recognizes C-terminal, internal, and N-terminal VSV-G-tag fusion proteins.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence of VSV-G-tag. The exact sequence is proprietary.
Description:
Mouse monoclonal antibody to VSV-G-tag
Uniprot:
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage:
Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
Data:
Western blot analysis of over-expressed VSV-G-tagged protein in 293T cell lysate.
Immunofluorescent analysis of VSV-G-tag staining in 293T cells transfected with a VSV-G-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation of VSV-G-tagged protein from HEK293T cells transfected with vector overexpressing VSV-G tag, using Anti-VSV-G-tag Antibody.
