Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:1000), IF/ICC (1:50 - 1:200)
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Zebrafish
Specificity:
Recognizes endogenous levels of SUMO2/3 protein.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SUMO2/3. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to SUMO2/3
Uniprot:
P61956
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
SUMO2; SMT3A; SMT3H2; Small ubiquitin-related modifier 2; SUMO-2; HSMT3; SMT3 homolog 2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3A; Smt3A
Data:
Western blot analysis of SUMO2/3 expression in HEK293T (A), A549 (B), U2OS (C), mouse brain (D), mouse testis (E), rat brain (F), rat testis (G) whole cell lysates. (Predicted band size: 10; 11 kD; Observed band size: 17 kD)
Immunofluorescent analysis of SUMO2/3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
