Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:1000), IF/ICC (1:50 - 1:200)
Reactivity:
Human
Specificity:
Recognizes endogenous levels of MRPL35 protein.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRPL35. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to MRPL35
Uniprot:
Q9NZE8
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
MRPL35; 39S ribosomal protein L35 mitochondrial; L35mt; MRP-L35
Data:
Western blot analysis of MRPL35 expression in HEK293T (A), Hela (B) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 25 kD)
Immunofluorescent analysis of MRPL35 staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
