Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:1000), IHC (1:100 - 1:200)
Reactivity:
Human
Specificity:
Recognizes endogenous levels of DZIP3 protein.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DZIP3. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to DZIP3
Uniprot:
Q86Y13
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
DZIP3; KIAA0675; E3 ubiquitin-protein ligase DZIP3; DAZ-interacting protein 3; RNA-binding ubiquitin ligase of 138 kDa; hRUL138
Data:
Western blot analysis of DZIP3 expression in HEK293T (A), LO2 (B) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 180; 139 kD)
Immunohistochemical analysis of DZIP3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
