Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:2000), IF/ICC (1:10 - 1:100)
Reactivity:
Human
Specificity:
Recognizes endogenous levels of CLIC1 protein.
Immunogen:
Recombinant full length protein of human CLIC1
Description:
Rabbit polyclonal antibody to CLIC1
Uniprot:
O00299
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
CLIC1; G6; NCC27; Chloride intracellular channel protein 1; Chloride channel ABP; Nuclear chloride ion channel 27; NCC27; Regulatory nuclear chloride ion channel protein; hRNCC
Data:
Western blot analysis of CLIC1 expression in Hela (A), HepG2 (B), mouse lung (C), rat kidney (D) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 27 kD)
Immunofluorescent analysis of CLIC1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
