Western blot analysis of CD249 expression in mouse lung (A) whole cell lysates. (Predicted band size: 109 kD; Observed band size: 109-160 kD)

Immunohistochemical analysis of CD249 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.