Western blot analysis of JAK2 (Phospho-Y221) expression in HepG2 (A), LO2 (B) whole cell lysates. (Predicted band size: 130 kD; Observed band size: 125 kD)
Immunohistochemical analysis of JAK2 (Phospho-Y221) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.