Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:1000), IHC (1:50 - 1:200)
Reactivity:
Human, Mouse, Rat
Specificity:
Recognizes endogenous levels of BAGE3 protein.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAGE3. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to BAGE3
Uniprot:
Q86Y29
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
BAGE3; B melanoma antigen 3; Cancer/testis antigen 2.3; CT2.3
Data:
Western blot analysis of BAGE3 expression in mouse kidney (A), rat brain (B) whole cell lysates. (Predicted band size: 12 kD; Observed band size: 17 kD)
Immunohistochemical analysis of BAGE3 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
