Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:1000), IHC (1:50 - 1:100)
Reactivity:
Human, Mouse, Rat, Chicken
Specificity:
Recognizes endogenous levels of Paxillin protein only when phosphorylated at S272.
Immunogen:
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S272 of human Paxillin protein. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to Paxillin (Phospho-S272)
Uniprot:
P49023
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
PXN; Paxillin
Data:
Western blot analysis of Paxillin (Phospho-S272) expression in HEK293T (A), MCF7 (B) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 68 kD)
Immunohistochemical analysis of Paxillin (Phospho-S272) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
