Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:1000), IHC (1:50 - 1:200)
Reactivity:
Human
Specificity:
Recognizes endogenous levels of RFX2 protein.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RFX2. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to RFX2
Uniprot:
P48378
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
RFX2; DNA-binding protein RFX2; Regulatory factor X 2
Data:
Western blot analysis of RFX2 expression in A2780 (A) whole cell lysates. (Predicted band size: 79 kD; Observed band size: 80 kD)
Immunohistochemical analysis of RFX2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
