Western blot analysis of CD294 expression in HEK293T (A), HuT78 (B), rat spleen (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)

Immunohistochemical analysis of CD294 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.