Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:2000), IHC (1:50 - 1:200)
Reactivity:
Human, Mouse, Rat
Specificity:
Recognizes endogenous levels of Connexin 47 protein
Immunogen:
KLH-conjugated synthetic peptide of human Connexin 47. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to Connexin 47
Uniprot:
Q5T442
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
GJC2; GJA12; Gap junction gamma-2 protein; Connexin-46.6; Cx46.6; Connexin-47; Cx47; Gap junction alpha-12 protein
Data:
Western blot analysis of Connexin 47 expression in mouse brain (A) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 50 kD)
Immunohistochemical analysis of Connexin 47 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
