Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB (1:500 - 1:1000), FC (1:10 - 1:50)
Reactivity:
Human
Specificity:
Recognizes endogenous levels of ANKRD40 protein.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human ANKRD40. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to ANKRD40
Uniprot:
Q6AI12
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% Thimerosal.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
ANKRD40; Ankyrin repeat domain-containing protein 40
Data:
Western blot analysis of ANKRD40 expression in 293 (A), HL60 (B), MCF7 (C) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 41 kD)
Flow cytometric analysis of HL60 cells using Anti-ANKRD40 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
