Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Thimerosal and 0.05% BSA.
Storage:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
SQSTM1; ORCA; OSIL; Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62
Data:
Western blot analysis of p62 expression in A549 (A), HL60 (B), U2OS (C) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 62 kD)
Immunofluorescent analysis of p62 staining in U87MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
