Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB: 1:500 - 1:1000
IHC: 1:100-1:200
Reactivity:
Human,Mouse,Rat
Specificity:
Recognizes endogenous levels of ATP5JL protein.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATP5JL. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to ATP5JL
Uniprot:
P56134
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage:
Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
ATP5J2; ATP synthase f chain mitochondrial; ATP5JL; ATPK
Data:
Western blot analysis of ATP5JL expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
Immunohistochemical analysis of ATP5JL staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
