Purification:The antibody was purified by immunogen affinity chromatography.
Useful information
Applications:
WB: 1:500 - 1:1000
IHC: 1:100-1:200
Reactivity:
Human
Specificity:
Recognizes endogenous levels of BAD (pS112) protein.
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAD (pS112). The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to BAD (pS112)
Uniprot:
Q92934
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage:
Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
BAD; BBC6; BCL2L8; Bcl2 antagonist of cell death; BAD; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-XL/Bcl-2-associated death promoter
Data:
Western blot analysis of BAD (phospho-S112) expression in HeLa Forskolin-treated (A), MCF7 (B) whole cell lysates.
Immunohistochemical analysis of BAD (phospho-S112) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
