KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL2. The exact sequence is proprietary.
Description:
Rabbit polyclonal antibody to BCL2
Uniprot:
P10415
BiowMW:
Refer to Figures
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage:
Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles.
Note:
For research use only, not for use in diagnostic procedure.
Alternative Name:
BCL2; Apoptosis regulator Bcl-2
Data:
Western blot analysis of BCL2 expression in HeLa (A), A2780 (B) whole cell lysates.
Immunohistochemical analysis of BCL2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of BCL2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
